« Gearing up the Right Thought Process for Solving Problems | Main | Identifying Peak Overlap on an HMBC Spectrum … Part 2 »

April 28, 2009

Comments

Feed You can follow this conversation by subscribing to the comment feed for this post.

marto

I am getting a singlet around 8.4 ppm for my compound. This compound was purified by Prep HPLC using Amm. Carboanate+CH3CN+H2O. Please tell me whether this peak could correspond to ammonium ions..Please tell me how to check this peak corresponds to ammonium ion? Thanks..

Arvin

Hello Marto,

If a 1H signal is attributed to an exchangeable proton, then adding some D2O to the sample will cause the signal to drop in intensity when collecting a second 1H NMR spectrum.

Furthermore, if you have some extra time on the NMR instrument, then a 1H-15N HSQC might be worth a try.

Below are some references that discuss NMR and ammonium ions:

Ref1 J. Am. Chem. Soc.,1961,v.83,p.4726
Ref2 Chem. Phys. Lett.,1988,v.143,p.471
Ref3 Magn. Reson. Chem.,2008,v.46,p.356
Ref4 J. Chem. Soc. (A),1968,p.1535
Ref5 S. Berger, S. Braun, H.-O. Kalinowski. NMR Spectroscopy of the Nonmetallic Elements (John Wiley & Sons, Chichester, 1997).,p.245
Ref6 Magn. Reson. Chem.,2008,v.46,p.356

marto

Thanks very much Arvin for the help.

Verify your Comment

Previewing your Comment

This is only a preview. Your comment has not yet been posted.

Working...
Your comment could not be posted. Error type:
Your comment has been saved. Comments are moderated and will not appear until approved by the author. Post another comment

The letters and numbers you entered did not match the image. Please try again.

As a final step before posting your comment, enter the letters and numbers you see in the image below. This prevents automated programs from posting comments.

Having trouble reading this image? View an alternate.

Working...

Post a comment

Comments are moderated, and will not appear until the author has approved them.

Your Information

(Name is required. Email address will not be displayed with the comment.)