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« Gearing up the Right Thought Process for Solving Problems | Main | Identifying Peak Overlap on an HMBC Spectrum … Part 2 »

April 28, 2009


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I am getting a singlet around 8.4 ppm for my compound. This compound was purified by Prep HPLC using Amm. Carboanate+CH3CN+H2O. Please tell me whether this peak could correspond to ammonium ions..Please tell me how to check this peak corresponds to ammonium ion? Thanks..


Hello Marto,

If a 1H signal is attributed to an exchangeable proton, then adding some D2O to the sample will cause the signal to drop in intensity when collecting a second 1H NMR spectrum.

Furthermore, if you have some extra time on the NMR instrument, then a 1H-15N HSQC might be worth a try.

Below are some references that discuss NMR and ammonium ions:

Ref1 J. Am. Chem. Soc.,1961,v.83,p.4726
Ref2 Chem. Phys. Lett.,1988,v.143,p.471
Ref3 Magn. Reson. Chem.,2008,v.46,p.356
Ref4 J. Chem. Soc. (A),1968,p.1535
Ref5 S. Berger, S. Braun, H.-O. Kalinowski. NMR Spectroscopy of the Nonmetallic Elements (John Wiley & Sons, Chichester, 1997).,p.245
Ref6 Magn. Reson. Chem.,2008,v.46,p.356


Thanks very much Arvin for the help.

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